You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Some proteins of the input proteomes never reach a rootHOG FASTA (and so no output file), without any warning. Unlike #113 this has not been quantified on fungi-30 yet.
merge_rhogs2 overflow: when a merge cluster exceeds --big-rhog-size, newhost is not reset between clusters and the overflow host loses its own proteins (_utils_roothog.py:667-713). Also, if all members of a cluster are gone, the previous cluster's host_hog is reused.
Proteins without any hogmap row are dropped (parse_hogmap_omamer, _utils_roothog.py:264). They are neither mapped nor in unmapped, so they don't reach linclust either.
Ids longer than 230 characters are truncated (_utils_roothog.py:256) and then no longer match the hogmap qseqid (:289). check-input rejects ids longer than 60 characters, so this only happens when check-input is bypassed; the two limits should agree anyway.
Minor isoforms reach linclust: isoform selection filters the hogmaps but not the unmapped proteins, so non-selected isoforms are clustered (infer_roothogs.py:50-60, :93).
Fix the four cases, and add an accounting step at the end of fastoma-infer-roothogs: every protein of the proteomes (after length filtering and isoform selection) is either in a rootHOG FASTA, in singleton_unmapped.fa, or reported as a deliberately dropped singleton, and the counts are logged.
Activity